Translocation of cell penetrating peptide engrafted nanoparticles across skin layers
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چکیده
منابع مشابه
Translocation of the cell-penetrating Tat peptide across artificial bilayers and into living cells.
The ability of a short, charged peptide to penetrate synthetic DOPC (1,2-dioleoyl-sn-3-glycerophosphocholine) liposomes was investigated by fluorescence confocal microscopy. The peptide, termed Tat (trans-activating transcription factor), was a 14-mer derived from the region of the HIV-1 Tat protein responsible for cellular internalization. This Tat peptide was labelled at a C-terminal cysteine...
متن کاملEvaluation of Cell Penetrating Peptide Delivery System on HPV16E7 Expression in Three Types of Cell Line
Background: The poor permeability of the plasma and nuclear membranes to DNA plasmids are two major barriers for the development of these therapeutic molecules. Therefore, success in gene therapy approaches depends on the development of efficient and safe non-viral delivery systems. Objectives: The aim of this study was to investigate the in vitro delivery of plasmid DNA encoding HPV16 E7 gene...
متن کاملTranslocation of a beta-peptide across cell membranes.
Short cationic peptides derived from DNA-binding proteins, of which HIV Tat is a prototype, can cross the membranes of living cells, and they can bring covalently attached moieties (proteins, drugs) along with them. We show that a beta-amino acid analogue of Tat 47-57 enters HeLa cells with comparable efficiency to Tat 47-57 itself (YGRKKRRQRRR). The beta-peptide is comprised of residues that b...
متن کاملLow concentration thresholds of plasma membranes for rapid energy-independent translocation of a cell-penetrating peptide.
The exact mechanisms by which cell-penetrating peptides such as oligo-arginines and penetratin cross biological membranes has yet to be elucidated, but this is required if they are to reach their full potential as cellular delivery vectors. In the present study, qualitative and quantitative analysis of the influence of temperature, peptide concentration and plasma membrane cholesterol on the up...
متن کاملCovalent Pyrenebutyrate-Cell Penetrating Peptide Conjugates: Enhanced Direct Membrane Translocation of Coupled Molecular Imaging Agents
SUMMARY: Intracellular targeting with cell penetrating peptides (CPPs) is limited by endocytotic mechanism of uptake. Here, we report that the well known CPP, dTat, chemically coupled with pyrenebutyrate (PB) can efficiently deliver directly into the cytosol. Results indicate that this combination could successfully translocate magnetic resonance imaging (MRI) agents into cytosol. INTRODUCTION:...
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ژورنال
عنوان ژورنال: Biomaterials
سال: 2010
ISSN: 0142-9612
DOI: 10.1016/j.biomaterials.2010.03.010